Daniel:Notebook/ComboLock/2017-1-10: Difference between revisions

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=Title=
=Version 2 Oligos (Started [[Daniel:Notebook/ComboLock/2017-1-6|1/6/2017]])=


[[Daniel:Notebook/ComboLock|Back to Calendar]]
[[Daniel:Notebook/ComboLock|Back to Calendar]]


ng/uL:40.3
==Library Prep (Started [[Daniel:Notebook/ComboLock/2017-1-9|Yesterday]])==
 
<ol start="2">
<li>Ethanol Precipitation</li>
<ol type="A" start="2">
<li>Centrifuge for 25 minutes at 12000 rpm at 4C</li>
<li>Remove supernatant and add 750 uL chilled 70% EtOH</li>
<li>Centrifuge for 12 minutes at 12000 rpm at 4C</li>
<li>Remove supernatant and spin in vacuum centrifuge for 10 minutes</li>
<li>Add 30 uL nfH2O to resuspend; pool samples A and B by reusing the water</li>
</ol></ol>
 
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="background-color:#C4BD97;font-size:12pt;font-weight:bold" align="center"
| width="160" height="34" | Sample
| width="85" | ng/uL
| width="85" | ug in 30 uL
 
|- style="font-size:12pt"
| height="30"  valign="bottom" | Cells RCA Product (2AB,2BB)
| align="center" align="center" | 47.8
| align="center" align="center" | 1.4
|}
 
'''DEJ20170109-CLv2-PC'''
 
ng/uL: 40.3

Revision as of 21:53, 13 January 2017

Version 2 Oligos (Started 1/6/2017)

Back to Calendar

Library Prep (Started Yesterday)

  1. Ethanol Precipitation
    1. Centrifuge for 25 minutes at 12000 rpm at 4C
    2. Remove supernatant and add 750 uL chilled 70% EtOH
    3. Centrifuge for 12 minutes at 12000 rpm at 4C
    4. Remove supernatant and spin in vacuum centrifuge for 10 minutes
    5. Add 30 uL nfH2O to resuspend; pool samples A and B by reusing the water
Sample ng/uL ug in 30 uL
Cells RCA Product (2AB,2BB) 47.8 1.4

DEJ20170109-CLv2-PC

ng/uL: 40.3