Daniel:Notebook/ComboLock/2017-2-10: Difference between revisions

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>Djacobse
>Djacobse
Line 15: Line 15:
| width="85" | No X
| width="85" | No X
| width="190" | Primer Pair
| width="190" | Primer Pair
| width="82" | Forward Primer Amt (10 uM)
| width="92" | Forward Primer Amt (10 uM)
| width="80" | Reverse Primer Amt (10 uM)
| width="90" | Reverse Primer Amt (10 uM)
| width="65" | uL nfH2O
| width="65" | uL nfH2O
| width="65" | uL 2X Kapa SYBR Fast
| width="95" | uL 2X Kapa SYBR Fast
| width="65" | Total
| width="65" | Total



Revision as of 01:15, 10 February 2017

Beads Binding Test (Started Yesterday)

Back to Calendar

Protocol-Part 2-qPCR

  1. qPCR
    1. Make qPCR master mixes according to following recipes
    2. Master Mix No X Primer Pair Forward Primer Amt (10 uM) Reverse Primer Amt (10 uM) uL nfH2O uL 2X Kapa SYBR Fast Total
      MMC1 73 Primer24/Primer4RC 73 73 1168 1460 2774
      MMC2 2.2 Primer6/Primer12RC 2.2 2.2 35.2 44 83.6
      MMT 2.2 PCCTAmpF/PCCT-AmpR 2.2 2.2 35.2 44 83.6
    3. Add 48 uL master mix to each well
    4. Add 2 uL sample according to table in following section
    5. qPCR Cycles
      1. 95C 3 min
      2. 95C 3 sec
      3. 55C 30 sec
      4. 72C 15 sec
      5. plate read
      6. goto b x24
      7. 72C 2 min
      8. 16C hold

    Plate Layouts

    File:20170209-Plate1-5.png

    File:20170209-Plate6-10.png