Daniel:Notebook/PosSequencing/2017-4-12: Difference between revisions
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>Djacobse (Created page with "== ==Bead Loading Test== <ol> <li>Oil phase</li> <ol type="A"> <li>Start from yesterday's oil prep (Span80-4.5%, Tween20-0.1%, T...") |
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== | =Emulsion Test-Variables (Started [[Daniel:Notebook/PosSequencing/2017-4-11|Yesterday]])= | ||
[[Daniel:Notebook/PosSequencing|Back to Calendar]] | |||
==Bead Loading Test== | ==Bead Loading Test== |
Revision as of 20:03, 12 April 2017
Emulsion Test-Variables (Started Yesterday)
Bead Loading Test
- Oil phase
- Start from yesterday's oil prep (Span80-4.5%, Tween20-0.1%, TritonX100-0.05% in mineral oil)
- Oil/aqueous mixing (adopted from Shao/Church-adjusted for Church protocol bead concentration)
- Mix 95 uL TE buffer and 10 uL magnetic Dynabeads; vortex to mix
- Mix 20 uL aqueous into 200 uL oil mixture
- Vortex for 30 seconds
- Repeat mixing to 100 uL (5 times)
- Mix with vortexer for 3 minutes
- Dilute with pure mineral oil for imaging (1:100)