AlanFung:Protocol/Cell Counting: Difference between revisions

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  Fill small cup with 10mL balance solution
  Fill small cup with 10mL balance solution
  Replace cleaning solution cup with
  Replace cleaning solution cup with
*Prepare cups with 10ml balance solution(1cup/sample)
*Prepare single cell suspension by repeat pipetting
*Aspirate 100uL of single cell suspension into cup
*

Revision as of 01:01, 17 June 2009

Cell Counting

  • Material
Cell counter
Small cup
Balance solution
5mL pipette
Pipette aid
P200 pipette
P200 pipette tips
  • Overview
Prime Amperture
Suspend Cells
Perform Cell Count
  • Protocol

Prime Amperature

Turn on cell counter
Fill small cup with 10mL balance solution
Replace cleaning solution cup with
  • Prepare cups with 10ml balance solution(1cup/sample)
  • Prepare single cell suspension by repeat pipetting
  • Aspirate 100uL of single cell suspension into cup