Daniel:Notebook/PosSequencing/2017-4-27: Difference between revisions
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[[Category: | [[Category:PosSeq]] [[Category:20170426]] |
Revision as of 00:17, 27 April 2017
Emulsion PCR (Started Yesterday)
Protocol Part 3-Hybridization and Imaging
- Fluorescent Oligo Binding
- Put suspension on magnet
- Resuspend beads in 25 uL 4X SSC buffer
- Add 25 uL fluorescent probe in 60% formamide
- Wash three times in 2X SSC
- Imaging