Daniel:Notebook/PosSequencing/2017-4-27: Difference between revisions
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==Protocol Part 3-Hybridization and Imaging== | ==Protocol Part 3-Hybridization and Imaging== | ||
Probe hybridization and washes based on [[Matt:LabNotes/2015-3-12|Matt's experiment]]. Calculations for dye binding: | |||
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<li>Resuspend beads in 25 uL 4X SSC buffer</li> | <li>Resuspend beads in 25 uL 4X SSC buffer</li> | ||
<li>Add 25 uL fluorescent probe in 60% formamide</li> | <li>Add 25 uL fluorescent probe in 60% formamide</li> | ||
<li>Incubate at room temp in the dark for 30 min</li> | |||
<li>Wash three times in 2X SSC</li> | <li>Wash three times in 2X SSC</li> | ||
</ol> | </ol> |
Revision as of 15:48, 27 April 2017
Emulsion PCR (Started Yesterday)
Protocol Part 3-Hybridization and Imaging
Probe hybridization and washes based on Matt's experiment. Calculations for dye binding:
- Fluorescent Oligo Binding
- Put suspension on magnet
- Resuspend beads in 25 uL 4X SSC buffer
- Add 25 uL fluorescent probe in 60% formamide
- Incubate at room temp in the dark for 30 min
- Wash three times in 2X SSC
- Imaging