Daniel:Notebook/PosSequencing/2017-4-27: Difference between revisions
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Probe hybridization and washes based on [[Matt:LabNotes/2015-3-12|Matt's experiment]]. Calculations for dye binding: | Probe hybridization and washes based on [[Matt:LabNotes/2015-3-12|Matt's experiment]]. Calculations for dye binding: | ||
α < | α 50×10<sup>-3</sup>×500pmol⁄mg | ||
Revision as of 15:55, 27 April 2017
Emulsion PCR (Started Yesterday)
Protocol Part 3-Hybridization and Imaging
Probe hybridization and washes based on Matt's experiment. Calculations for dye binding:
α 50×10-3×500pmol⁄mg
- Fluorescent Oligo Binding
- Put suspension on magnet
- Resuspend beads in 25 uL 4X SSC buffer
- Add 25 uL fluorescent probe in 60% formamide
- Incubate at room temp in the dark for 30 min
- Wash three times in 2X SSC
- Imaging