Daniel:Notebook/PosSequencing/2017-4-28: Difference between revisions
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>Djacobse (Created page with "=Emulsion PCR (Started Yesterday)= Back to Calendar ==Images and Analysis== [[Category:Pos...") |
>Djacobse |
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==Images and Analysis== | ==Images and Analysis== | ||
<gallery perrow=3 heights=300px widths=300px caption="Field 4 Images"> | |||
File:20170428-emPCR2_w2GFP4.jpg|Alexa 488; barcode oligo v1 | |||
File:20170428-emPCR2_w4Cy3-4.jpg|Cy3 image (Note: No Cy3 was used, therefore there shouldn't be a Cy3 signal) | |||
File:20170428-emPCR2_w3Cy5-4.jpg|Cy5 image; barcode oligo v3 | |||
File:2017-04-28-emPCR-Field4-Overlay-Cy3-GFP-Cy5.jpg|Dye overlays; Cy3-white; A488-green; Cy5-Red | |||
File:20170428-Field4Overlay-Measured.png|Dye overlays, individual bubbles measured as 2.5 um each (2 A488, 1 Cy5) | |||
</gallery> | |||
[[Category:PosSeq]] [[Category:20170426]] | [[Category:PosSeq]] [[Category:20170426]] |
Revision as of 19:18, 28 April 2017
Emulsion PCR (Started Yesterday)
Images and Analysis
- Field 4 Images
- 20170428-emPCR2 w2GFP4.jpg
Alexa 488; barcode oligo v1
- 20170428-emPCR2 w4Cy3-4.jpg
Cy3 image (Note: No Cy3 was used, therefore there shouldn't be a Cy3 signal)
- 20170428-emPCR2 w3Cy5-4.jpg
Cy5 image; barcode oligo v3
- 2017-04-28-emPCR-Field4-Overlay-Cy3-GFP-Cy5.jpg
Dye overlays; Cy3-white; A488-green; Cy5-Red
- 20170428-Field4Overlay-Measured.png
Dye overlays, individual bubbles measured as 2.5 um each (2 A488, 1 Cy5)