Daniel:Protocols/AmiconUltra: Difference between revisions

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Amicon Ultra Centrifugal filters are microcentrifuge filters that allow you to purify and buffer exchange various MW cutoff biomacromolecules. They use a MW cutoff filter, say 10 kDa, to allow dialysate through while not passing the biomacromolecules. They can be used for buffer exchange via the following general protocol:
Amicon Ultra Centrifugal filters are microcentrifuge filters that allow you to purify and buffer exchange various MW cutoff biomacromolecules. They use a MW cutoff filter, say 10 kDa, to allow dialysate through while not passing the biomacromolecules. They can be used for buffer exchange via the following general protocol:


[[Image:CentrifugalFilters.png|400px]]
<gallery perrow=2 heights=300px widths=400px>
File:CentrifugalFilters-Purification.png|Purification/Concentration
File:CentrifugalFilters.png|Buffer Exchange
</gallery>


==Protocol-Filtration==
==Protocol-Filtration==

Revision as of 21:44, 13 May 2017

Amicon Ultra Centrifugal Filters

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Amicon Order Page/Information

Amicon Ultra Centrifugal filters are microcentrifuge filters that allow you to purify and buffer exchange various MW cutoff biomacromolecules. They use a MW cutoff filter, say 10 kDa, to allow dialysate through while not passing the biomacromolecules. They can be used for buffer exchange via the following general protocol:

Protocol-Filtration

  1. Add X uL sample and buffer to 500 uL total volume
  2. Spin down at 14000 x g for appropriate time (15 minutes for 22 uL volume)
  3. Discard flow through tube and get a new one
  4. Invert filter inside flow through tube and spin down at 14000xg for 1 minute
  5. Alternately, extract sample using pipette

Protocol-Dialysis

  1. Add X uL sample and new buffer to 500 uL total volume
  2. Spin down at 14000 x g for appropriate time (15 minutes for 22 uL volume)
  3. Discard flow through and add 500 uL new buffer
  4. Repeat steps 2 and 3 as many times as desired