Alice:LabNotes/2007-12-12: Difference between revisions
Jump to navigation
Jump to search
>Zsakura2 No edit summary |
>Zsakura2 No edit summary |
||
Line 11: | Line 11: | ||
|- | |- | ||
| post-PCR sample ||0 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul || | | post-PCR sample ||0 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul || | ||
<b>Note: there were two very serious mistakes on this experiment: first of all, I did not add hot-start enzyme to the reaction, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. Second, the reaction was not set up on ice. | <b>Note: there were two very serious mistakes on this experiment: first of all, I did not add hot-start enzyme to the reaction, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. Second, the reaction was not set up on ice. | ||
[[Image:ZhangLab_2 2007-12-12 13hr 32min.jpg]] | [[Image:ZhangLab_2 2007-12-12 13hr 32min.jpg]] |
Revision as of 07:43, 26 December 2007
Agarose gel on post-PCR product of PGP1Ver3 Primers
' | well 1 | well 2-13 | |||||||
Low Mass bp Ladder | 3 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
Novex 5x TBE loading dye | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | |
post-PCR sample | 0 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul |
Note: there were two very serious mistakes on this experiment: first of all, I did not add hot-start enzyme to the reaction, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. Second, the reaction was not set up on ice. File:ZhangLab 2 2007-12-12 13hr 32min.jpg |