Alice:LabNotes/2007-12-12: Difference between revisions
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| post-PCR sample ||0 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul || | | post-PCR sample ||0 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul || | ||
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[[Image:ZhangLab_2 2007-12-12 13hr 32min.jpg]] | [[Image:ZhangLab_2 2007-12-12 13hr 32min.jpg]] | ||
<b>Note: there were two very serious mistakes on this experiment: first of all, I did not add hot-start enzyme to the reaction, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. Second, the reaction was not set up on ice. |
Revision as of 07:44, 26 December 2007
Agarose gel on post-PCR product of PGP1Ver3 Primers
' | well 1 | well 2-13 | |||||||
Low Mass bp Ladder | 3 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
Novex 5x TBE loading dye | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | 2 ul | |
post-PCR sample | 0 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | 3 ul | |
File:ZhangLab 2 2007-12-12 13hr 32min.jpg
Note: there were two very serious mistakes on this experiment: first of all, I did not add hot-start enzyme to the reaction, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. Second, the reaction was not set up on ice.