AlanFung:Protocol/OliGreen ssDNA Stain: Difference between revisions
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*20X TE Buffer (Component B) | *20X TE Buffer (Component B) | ||
*Oligonucleotide Standard (Component C) 18-base M13 sequence primer, | *Oligonucleotide Standard (Component C) 18-base M13 sequence primer, | ||
with the sequence 5'-TGTAAAACGACGGCCAGT-3' | |||
*Sterile, distilled, DNase-free water | *Sterile, distilled, DNase-free water | ||
*96 well microplates | *96 well microplates |
Revision as of 18:14, 28 May 2009
Probe Preparation
Samples & Materials
- Quant-iT OliGreen ss DNA Assay Kit (Cat. no. O11492)
*Quant-iT OliGreen ss DNA Reagent (Component A), solution indimethylsulfoxide (DMSO) *20X TE Buffer (Component B) *Oligonucleotide Standard (Component C) 18-base M13 sequence primer, with the sequence 5'-TGTAAAACGACGGCCAGT-3'
- Sterile, distilled, DNase-free water
- 96 well microplates
- Microplate Reader
Procedure
Allow