AlanFung:Protocol/OliGreen ssDNA Stain: Difference between revisions
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*Microplate Reader | *Microplate Reader | ||
== | ==Overview== | ||
Allow | |||
==Procedures== | |||
==Step 1: Preparing Reagent== | |||
*Allow the reagent to warm to RT before opening the vial | |||
*Prepare an aqueous working solution of the Quant-iT OliGreen reagent by making a 200 fold dilution of the concentrated DMSO solution in 10nM Tris-HCL | |||
1mM EDTA | |||
pH 7.5 (TE) | |||
==Step 2: Oligonucleotide Standard Curve== |
Revision as of 18:19, 28 May 2009
Probe Preparation
Samples & Materials
- Quant-iT OliGreen ss DNA Assay Kit (Cat. no. O11492)
*Quant-iT OliGreen ss DNA Reagent (Component A), solution indimethylsulfoxide (DMSO) *20X TE Buffer (Component B) *Oligonucleotide Standard (Component C) 18-base M13 sequence primer, with the sequence 5'-TGTAAAACGACGGCCAGT-3'
- Sterile, distilled, DNase-free water
- 96 well microplates
- Microplate Reader
Overview
Procedures
Step 1: Preparing Reagent
- Allow the reagent to warm to RT before opening the vial
- Prepare an aqueous working solution of the Quant-iT OliGreen reagent by making a 200 fold dilution of the concentrated DMSO solution in 10nM Tris-HCL
1mM EDTA pH 7.5 (TE)