Kun:LabNotes/ExonomeSeq/2007-12-17: Difference between revisions

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(New page: ==Exp.1 Received the Agilent library today, start to make probes== PCR (with NEB Taq master mix) x1 x32 E55k1(20nM) 0.1ul ...)
 
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==Exp.1 Received the Agilent library today, start to make probes==
==Exp.1 Start another round of E55kv1 probe production==
PCR (with NEB Taq master mix)
PCR (with NEB Taq master mix)
                               x1          x32     
                               x1          x32     

Revision as of 00:01, 18 December 2007

Exp.1 Start another round of E55kv1 probe production

PCR (with NEB Taq master mix)

                             x1          x32     
        E55k1(20nM)         0.1ul       3.2ul    
        2x Taq master mix    50ul      1600ul   
        100uM pAP1V6U       0.3ul       9.6ul   
        100uM phosAP2V6     0.3ul       9.6ul   
        H2O                49.3ul      1600ul    

94C 3min -> 18 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 15C hold