Kun:LabNotes/ExonomeSeq/2007-12-17: Difference between revisions

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==Exp.1 Start another round of E55kv1 probe production==
==Exp.1 Start another round of E55kv1 probe production==
PCR (with NEB Taq master mix)
PCR (with NEB Taq master mix)
                               x1          x32      
                               x1          x96      
         E55k1(20nM)        0.1ul      3.2ul    
         E55k1(20nM)        0.1ul      9.6ul    
         2x Taq master mix    50ul      1600ul    
         2x Taq master mix    50ul      4800ul    
         100uM pAP1V6U      0.3ul       9.6ul    
         100uM pAP1V6U      0.3ul     28.8ul    
         100uM phosAP2V6    0.3ul       9.6ul    
         100uM phosAP2V6    0.3ul     28.8ul    
         H2O                49.3ul      1600ul    
         H2O                49.3ul      4800ul    


94C 3min -> 18 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 15C hold
94C 3min -> 18 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 15C hold

Revision as of 00:03, 18 December 2007

Exp.1 Start another round of E55kv1 probe production

PCR (with NEB Taq master mix)

                             x1          x96     
        E55k1(20nM)         0.1ul       9.6ul    
        2x Taq master mix    50ul      4800ul   
        100uM pAP1V6U       0.3ul      28.8ul   
        100uM phosAP2V6     0.3ul      28.8ul   
        H2O                49.3ul      4800ul    

94C 3min -> 18 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 15C hold