Kun:LabNotes/ExonomeSeq/2007-12-17: Difference between revisions
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==Exp.1 Start another round of E55kv1 probe production== | ==Exp.1 Start another round of E55kv1 probe production== | ||
PCR (with NEB Taq master mix) | PCR (with NEB Taq master mix) | ||
x1 | x1 x96 | ||
E55k1(20nM) 0.1ul | E55k1(20nM) 0.1ul 9.6ul | ||
2x Taq master mix 50ul | 2x Taq master mix 50ul 4800ul | ||
100uM pAP1V6U 0.3ul | 100uM pAP1V6U 0.3ul 28.8ul | ||
100uM phosAP2V6 0.3ul | 100uM phosAP2V6 0.3ul 28.8ul | ||
H2O 49.3ul | H2O 49.3ul 4800ul | ||
94C 3min -> 18 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 15C hold | 94C 3min -> 18 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 15C hold |
Revision as of 00:03, 18 December 2007
Exp.1 Start another round of E55kv1 probe production
PCR (with NEB Taq master mix)
x1 x96 E55k1(20nM) 0.1ul 9.6ul 2x Taq master mix 50ul 4800ul 100uM pAP1V6U 0.3ul 28.8ul 100uM phosAP2V6 0.3ul 28.8ul H2O 49.3ul 4800ul
94C 3min -> 18 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 15C hold