Kun:LabNotes/ExonomeSeq/2007-12-23: Difference between revisions
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(New page: ==Exp.1 PCR on the capturing reaction started on 12/21== Template: 10ul 10X JumpStart buffer: 9ul 25mM dNTP 1ul 100uM AmpFV6.2Sol 0.4ul...) |
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Line 6: | Line 6: | ||
100uM AmpRV6.2Sol 0.4ul | 100uM AmpRV6.2Sol 0.4ul | ||
50X SYBG I 0.2ul | 50X SYBG I 0.2ul | ||
JumpStart Taq 1ul | |||
H2O 79ul | H2O 79ul | ||
94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold. | 94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold. |
Revision as of 01:21, 24 December 2007
Exp.1 PCR on the capturing reaction started on 12/21
Template: 10ul 10X JumpStart buffer: 9ul 25mM dNTP 1ul 100uM AmpFV6.2Sol 0.4ul 100uM AmpRV6.2Sol 0.4ul 50X SYBG I 0.2ul JumpStart Taq 1ul H2O 79ul
94C 3min -> (94C 45sec -> 56C 1min -> 72C 1min) x 8 -> (94C 30sec -> 72C 1min) x 10 -> 72C 3min -> 4C hold.