Kun:LabNotes/CpgSeq/2007-12-30: Difference between revisions

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     [[Image:2007-12-30-CpG-PAGE-PCR.png|400px]]
     [[Image:2007-12-30-CpG-PAGE-PCR.png|400px]]
     [[Image:2007-12-30-CpG-PAGE-PCR-gel.png|400px]]
     [[Image:2007-12-30-CpG-PAGE-PCR-gel.png|400px]]
I don't think the CpGMIPs captured any sequences from the bisulfite converted genome. Perhaps there wasn't too much DNA? Or the gap is too long?

Latest revision as of 02:12, 31 December 2007

Exp.1 Perform PCR on the gel size selected fragment from the 12-23-07 PCR products[edit]

   Into 100ul of eluted DNA, add:
   25mM dNTP:     0.6ul
   100uM AmpSolF: 0.2ul
   100uM AmpSolR: 0.2ul
   JumpStart Taq: 0.5ul
   50x SYBG I:    0.2ul

94C 3min -> (94C 30sec -> 72C 30sec) x 18 -> 72C 3min -> 4C hold.

   Real-time Curves
   File:2007-12-30-CpG-PAGE-PCR.png
   File:2007-12-30-CpG-PAGE-PCR-gel.png

I don't think the CpGMIPs captured any sequences from the bisulfite converted genome. Perhaps there wasn't too much DNA? Or the gap is too long?