Jie:LabNotes/CpgSeq/2009-6-22: Difference between revisions
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The primers are 20061015_ASM_valid. I set the annealing temperature at 58C, 62C. | The primers are 20061015_ASM_valid. I set the annealing temperature at 58C, 62C. | ||
96C 3min-> 40x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min. | 96C 3min-> 40x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min. | ||
[[Image:20090622_ASM_58C anneal.jpg]]20090622_ASM_58C anneal | |||
[[Image:20090622_ASM_62C anneal.jpg]]20090622_ASM_62C anneal |
Revision as of 16:57, 23 June 2009
Methyl_PCR for ASE validation
PCR on bisuphite conversed Jurkat-DNA
2x iQ supermix 20 x 12 Primer F (50uM) 0.5 0.5 each Primer R (50uM) 0.5 0.5 each BS Jurkat gDNA (202ng/ul) 0.1 1.2 dH2O 20 240 total 40ul
The primers are 20061015_ASM_valid. I set the annealing temperature at 58C, 62C. 96C 3min-> 40x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min.
File:20090622 ASM 58C anneal.jpg20090622_ASM_58C anneal
File:20090622 ASM 62C anneal.jpg20090622_ASM_62C anneal