AlanFung:Protocol/Genome Analyzer: Difference between revisions

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(New page: ==Preparing Reagents for the Genome Analyzer== *Genome analyzer Reagent Compartment & Reagent Position Image:Reagent Position.jpg)
 
>Alan6017518
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*Genome analyzer Reagent Compartment & Reagent Position
*Genome analyzer Reagent Compartment & Reagent Position
[[Image:Reagent Position.jpg]]
[[Image:Reagent Position.jpg]]
*Position of each reagent in the Genome Analyzer
NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER
Position    Reagent              Volume              Container
1          Incorporation Mix    Varies w/ cycle #  50ml amber tube
2          DI H2O                                  259ml bottle
3          Scanning Mix        50ml                50ml tube
4          High Salt Buffer                        250ml bottle
5          Incorporation buffer 150ml              250ml bottle
6          Cleavage Mix        50ml                50ml tube
7          Cleavage Buffer      70ml                250ml bottle

Revision as of 18:21, 25 June 2009

Preparing Reagents for the Genome Analyzer

  • Genome analyzer Reagent Compartment & Reagent Position

File:Reagent Position.jpg

  • Position of each reagent in the Genome Analyzer
NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER
Position    Reagent              Volume              Container
1           Incorporation Mix    Varies w/ cycle #   50ml amber tube
2           DI H2O                                   259ml bottle
3           Scanning Mix         50ml                50ml tube
4           High Salt Buffer                         250ml bottle
5           Incorporation buffer 150ml               250ml bottle
6           Cleavage Mix         50ml                50ml tube
7           Cleavage Buffer      70ml                250ml bottle