AlanFung:Protocol/Genome Analyzer: Difference between revisions

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>Alan6017518
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*Position of each reagent in the Genome Analyzer
*Position of each reagent in the Genome Analyzer
  NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER
  NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER
  Position    Reagent             Volume              Container
  Position    Reagent               Volume              Container
  1          Incorporation Mix   Varies w/ cycle #  50ml amber tube
  1          Incorporation Mix     Varies w/ cycle #  50ml amber tube
  2          DI H2O                                   259ml bottle
  2          DI H2O                                     259ml bottle
  3          Scanning Mix         50ml                50ml tube
  3          Scanning Mix           50ml                50ml tube
  4          High Salt Buffer                         250ml bottle
  4          High Salt Buffer                           250ml bottle
  5          Incorporation buffer 150ml              250ml bottle
  5          Incorporation buffer   150ml              250ml bottle
  6          Cleavage Mix         50ml                50ml tube
  6          Cleavage Mix           50ml                50ml tube
  7          Cleavage Buffer     70ml                250ml bottle
  7          Cleavage Buffer       70ml                250ml bottle

Revision as of 18:21, 25 June 2009

Preparing Reagents for the Genome Analyzer

  • Genome analyzer Reagent Compartment & Reagent Position

File:Reagent Position.jpg

  • Position of each reagent in the Genome Analyzer
NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER
Position    Reagent                Volume              Container
1           Incorporation Mix      Varies w/ cycle #   50ml amber tube
2           DI H2O                                     259ml bottle
3           Scanning Mix           50ml                50ml tube
4           High Salt Buffer                           250ml bottle
5           Incorporation buffer   150ml               250ml bottle
6           Cleavage Mix           50ml                50ml tube
7           Cleavage Buffer        70ml                250ml bottle