AlanFung:Protocol/Genome Analyzer: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 |
>Alan6017518 |
||
Line 4: | Line 4: | ||
*Position of each reagent in the Genome Analyzer | *Position of each reagent in the Genome Analyzer | ||
NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER | NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER | ||
Position Reagent | Position Reagent Volume Container | ||
1 Incorporation Mix | 1 Incorporation Mix Varies w/ cycle # 50ml amber tube | ||
2 DI H2O | 2 DI H2O 259ml bottle | ||
3 Scanning Mix | 3 Scanning Mix 50ml 50ml tube | ||
4 High Salt Buffer | 4 High Salt Buffer 250ml bottle | ||
5 Incorporation buffer 150ml 250ml bottle | 5 Incorporation buffer 150ml 250ml bottle | ||
6 Cleavage Mix | 6 Cleavage Mix 50ml 50ml tube | ||
7 Cleavage Buffer | 7 Cleavage Buffer 70ml 250ml bottle |
Revision as of 18:21, 25 June 2009
Preparing Reagents for the Genome Analyzer
- Genome analyzer Reagent Compartment & Reagent Position
- Position of each reagent in the Genome Analyzer
NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER Position Reagent Volume Container 1 Incorporation Mix Varies w/ cycle # 50ml amber tube 2 DI H2O 259ml bottle 3 Scanning Mix 50ml 50ml tube 4 High Salt Buffer 250ml bottle 5 Incorporation buffer 150ml 250ml bottle 6 Cleavage Mix 50ml 50ml tube 7 Cleavage Buffer 70ml 250ml bottle