AlanFung:Protocil/Cluster Station: Difference between revisions

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>Alan6017518
(New page: ==='''Performing linearization, blocking , and primer hybridization'''=== ===wash the cluster station=== *select file|open recipe *open the standard protocol recipe and click run *install...)
 
>Alan6017518
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  wear gloves at all times do not touch reagents with bare hands
  wear gloves at all times do not touch reagents with bare hands
  connect the tubes by holding the caps stationary while you twist the tubes into place to prevent crimping and twisting of the lines
  connect the tubes by holding the caps stationary while you twist the tubes into place to prevent crimping and twisting of the lines
===perform linearization blocking and primer hybridization===
*load reagents in the following positions as indicated
single

Revision as of 14:36, 21 July 2009

Performing linearization, blocking , and primer hybridization

wash the cluster station

  • select file|open recipe
  • open the standard protocol recipe and click run
  • install the washing bridge and load containers filled with water in the following positions
*single-read recipe-load position  3,4,7,12,15,17 
*paired-end recipe-load position  3,4,7,12,14,17
  • click ok to proceed and follow the prompts when the wash is complete
  • remove water from the following positions
*single-read recipe-load position  3,4,7,12,15,17
*paired-end recipe-load position  3,4,7,12,14,17
  • click ok and follow the prompts when the air gap has been primed

loading reagents

best practices
always remove and replace reagents one tube at a time
wear gloves at all times do not touch reagents with bare hands
connect the tubes by holding the caps stationary while you twist the tubes into place to prevent crimping and twisting of the lines

perform linearization blocking and primer hybridization

  • load reagents in the following positions as indicated
single