AlanFung:Protocil/Cluster Station: Difference between revisions
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>Alan6017518 (New page: ==='''Performing linearization, blocking , and primer hybridization'''=== ===wash the cluster station=== *select file|open recipe *open the standard protocol recipe and click run *install...) |
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wear gloves at all times do not touch reagents with bare hands | wear gloves at all times do not touch reagents with bare hands | ||
connect the tubes by holding the caps stationary while you twist the tubes into place to prevent crimping and twisting of the lines | connect the tubes by holding the caps stationary while you twist the tubes into place to prevent crimping and twisting of the lines | ||
===perform linearization blocking and primer hybridization=== | |||
*load reagents in the following positions as indicated | |||
single |
Revision as of 14:36, 21 July 2009
Performing linearization, blocking , and primer hybridization
wash the cluster station
- select file|open recipe
- open the standard protocol recipe and click run
- install the washing bridge and load containers filled with water in the following positions
*single-read recipe-load position 3,4,7,12,15,17 *paired-end recipe-load position 3,4,7,12,14,17
- click ok to proceed and follow the prompts when the wash is complete
- remove water from the following positions
*single-read recipe-load position 3,4,7,12,15,17 *paired-end recipe-load position 3,4,7,12,14,17
- click ok and follow the prompts when the air gap has been primed
loading reagents
best practices always remove and replace reagents one tube at a time wear gloves at all times do not touch reagents with bare hands connect the tubes by holding the caps stationary while you twist the tubes into place to prevent crimping and twisting of the lines
perform linearization blocking and primer hybridization
- load reagents in the following positions as indicated
single