HUES: Difference between revisions

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>Alan6017518
>Alan6017518
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*2 x PMEFi
*2 x PMEFi
==Materials and Reagents==
==Materials and Reagents==
*KO-DMEM
{| {{table}}
*DMEM
| align="center" style="background:#f0f0f0;"|'''Materials / Catalogue no.  '''
*FBS
| align="center" style="background:#f0f0f0;"|''''''
*KO-Serum Replacement
| align="center" style="background:#f0f0f0;"|''''''
*Plasmanate
|-
*bFGF
|  ||||
*hLIF
|-
*2-beta-mercaptoethanol
| KO‐DMEM      || Invitrogen Gibco  ||  CAT#10829 
*NEAA
|-
*Pen/Strep
|  ||||
*Glutamax-I
|-
*Mitomycin
| DMEM       ||Invitrogen Gibco    ||CAT#11965‐092 
*DMSO
|-
*Gelatin
|  ||||
*Tissue Culture Treated Plates
|-
*Hygromycin resistant pre-treated MEFs
| FBS       Va||lley Biomedical, Inc||CAT#BS‐3033 
*Neomycin resistant pre-treated MEFs
|-
|  ||||
|-
| •||||
|-
| KO‐Serum Replacenment ||Invitrogen Gibco    ||CAT#10828 
|-
|  ||||
|-
| Plasmanate ||Bayer||CAT#NDC 0026‐0613‐20 
|-
|  ||||
|-
| bFGF       ||Invitrogen Gibco    ||CAT#13256‐029 
|-
|  ||||
|-
| hLIF       ||Chemicon International||CAT#LIF1010 
|-
|  ||||
|-
| 2‐beta‐mercaptoethanol||Gibco||CAT#21985‐023 
|-
|  ||||
|-
| Non‐essential amino acids (NEAA) ||Invitrogen Gibco    ||CAT#11140050 
|-
| (10mM or 100x)||||
|-
|  ||||
|-
| Pen/Strep   ||Invitrogen Gibco    ||CAT#15070‐063 
|-
| (5000U/ml Penicillin G sodium and|||| 
|-
| 5000ug/ml Streptomycin sulfate in 0.85% saline)  ||||
|-
|  ||||
|-
| Glutamax‐I  ||Invitrogen Gibco    ||CAT#35050‐061 
|-
| (200mM or 100x)  ||||
|-
|  ||||
|-
| Mitomycin C||Sigma    ||CAT#M‐0503 
|-
|  ||||
|-
| 0.05% Trypsin/EDTA||Invitrogen Gibco    ||CAT#25300‐054 
|-
|  ||||
|-
| Dimethyl sulphoxide (DMSO)||Sigma    ||CAT#D2650 
|-
|  ||||
|-
| Gelatin     ||Sigma      ||CAT#G1890 
|-
|  ||||
|-
| Tissue Culture Treated Plates (6 well)||Coring||CAT#3506
|-
|  ||||
|-
| Hygromycin resistant, pre-treated MEFs ||Specialty Media||CAT#PMEF‐H 
|-
|  ||||
|-
| Neomycin resistant, pre-treated MEFs||Specialty Media||CAT#PMEF‐N
|-
|
|}


==Contact Information==
==Contact Information==

Revision as of 15:54, 24 July 2009

HUES Cell Collection

HUES homepage

  • HUES homepage[1]

Protocol

Storage

  • Location:Dr. Varghese lab LN tank Rack #2 Box "Zhang Lab"

HUES Cell Log

  • 1 x HUES-3 p22
  • 1 x HUES-8 p19
  • 1 x HUES-53 p11
  • 1 x HUES-62 p10
  • 1 x HUES-64 p12
  • 1 x HUES-66 p12
  • 2 x PMEFi

Materials and Reagents

Materials / Catalogue no. ' '
KO‐DMEM Invitrogen Gibco CAT#10829
DMEM Invitrogen Gibco CAT#11965‐092
FBS Va lley Biomedical, Inc CAT#BS‐3033
KO‐Serum Replacenment Invitrogen Gibco CAT#10828
Plasmanate Bayer CAT#NDC 0026‐0613‐20
bFGF Invitrogen Gibco CAT#13256‐029
hLIF Chemicon International CAT#LIF1010
2‐beta‐mercaptoethanol Gibco CAT#21985‐023
Non‐essential amino acids (NEAA) Invitrogen Gibco CAT#11140050
(10mM or 100x)
Pen/Strep Invitrogen Gibco CAT#15070‐063
(5000U/ml Penicillin G sodium and
5000ug/ml Streptomycin sulfate in 0.85% saline)
Glutamax‐I Invitrogen Gibco CAT#35050‐061
(200mM or 100x)
Mitomycin C Sigma CAT#M‐0503
0.05% Trypsin/EDTA Invitrogen Gibco CAT#25300‐054
Dimethyl sulphoxide (DMSO) Sigma CAT#D2650
Gelatin Sigma CAT#G1890
Tissue Culture Treated Plates (6 well) Coring CAT#3506
Hygromycin resistant, pre-treated MEFs Specialty Media CAT#PMEF‐H
Neomycin resistant, pre-treated MEFs Specialty Media CAT#PMEF‐N

Contact Information

HUES Cell Facility Contact Information

  • HUES Facility / Melton Laboratory / HHMI
  • Harvard University
  • Cambridge, MA 02138
  • Fax: 617.384.9463
  • Please send all HUES cell correspondence to hues@mcb.harvard.edu.

Harvard's Office of Technology Development Contact Information

  • Harvard's Office of Technology Development
  • 1350 Massachusetts Avenue
  • Holyoke Center 727
  • Cambridge, MA 02138
  • Fax: 617.495.9568
  • Please send all correspondence concerning the MTA to Material Transfers: materialtransfer@harvard.edu.