Jie:LabNotes/CpgSeq/2009-7-27: Difference between revisions
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>Jie deng (New page: ==MMP9 ASE of PGP1F and PGP1L== ===PCR=== The primers x 6 2x iQ supermix 40 240 Primer F (100uM) ...) |
>Jie deng No edit summary |
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Line 3: | Line 3: | ||
The primers | The primers | ||
x | x 4 | ||
2x iQ supermix 40 | 2x iQ supermix 40 160 | ||
Primer F (100uM) 0.4 | Primer F (100uM) 0.4 1.6 each | ||
Primer R (100uM) 0.4 | Primer R (100uM) 0.4 1.6 each | ||
cDNA samples as above: 0.1 | cDNA samples as above: 0.1 | ||
dH2O 40 | dH2O 40 160 | ||
total 80ul | total 80ul | ||
96C 3min-> 45x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min. | 96C 3min-> 45x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min. |
Revision as of 18:40, 27 July 2009
MMP9 ASE of PGP1F and PGP1L
PCR
The primers x 4 2x iQ supermix 40 160 Primer F (100uM) 0.4 1.6 each Primer R (100uM) 0.4 1.6 each cDNA samples as above: 0.1 dH2O 40 160 total 80ul
96C 3min-> 45x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min.