Jie:LabNotes/CpgSeq/2009-7-27: Difference between revisions

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[[Image:20090727_PGP1F_PGP1L_MMP9_ASE.jpg|300px]]20090727_PGP1F_PGP1L_MMP9_ASE
[[Image:20090727_PGP1F_PGP1L_MMP9_ASE.jpg|300px]]20090727_PGP1F_PGP1L_MMP9_ASE


I sent PGP1F_62C and PGP1L_58C to do the sanger sequencing.
  The sanger sequencing results did not show ASE of MMP9 in two cell lines.
  The sanger sequencing results did not show ASE of MMP9 in two cell lines.

Revision as of 18:36, 28 July 2009

MMP9 ASE of PGP1F and PGP1L

PCR

The primers: MMP9_ASE_primer
                                           x 4
2x iQ supermix                40           160         
Primer F (100uM)             0.4           1.6 each
Primer R (100uM)             0.4           1.6 each
cDNA samples as above:       0.1	  	
dH2O                          40           160
total                         80ul

96C 3min-> 45x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min.

File:20090727 PGP1F PGP1L MMP9 ASE.jpg20090727_PGP1F_PGP1L_MMP9_ASE

I sent PGP1F_62C and PGP1L_58C to do the sanger sequencing.
The sanger sequencing results did not show ASE of MMP9 in two cell lines.