AlanFung:LabNotes/EZ/2009-8-14: Difference between revisions
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>Alan6017518 (New page: ='''EZ DNA Methylation Direct - GM20431 cells'''= ==Objective== *Extract DNA from 2000 GM20431 cells *Elute DNA with 8uL elution buffer *Use all 8uL dna as template for one pair of prim...) |
>Alan6017518 |
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*Perform serial dilution to obtain | *Perform serial dilution to obtain | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"| | | align="center" style="background:#f0f0f0;"|200ng methylated DNA | ||
| 100ng methylated DNA | | 100ng methylated DNA | ||
|- | |- |
Revision as of 19:25, 14 August 2009
EZ DNA Methylation Direct - GM20431 cells
Objective
- Extract DNA from 2000 GM20431 cells
- Elute DNA with 8uL elution buffer
- Use all 8uL dna as template for one pair of primer
- Quantify amount of DNA with Nanodrop Qubit and Gel quantification
- Perform serial dilution to obtain
200ng methylated DNA | 100ng methylated DNA |
50ng methylated DNA | |
30ng methylated DNA | |
10ng methylated DNA | |