AlanFung:LabNotes/EZ/2009-8-14: Difference between revisions

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>Alan6017518
(New page: ='''EZ DNA Methylation Direct - GM20431 cells'''= ==Objective== *Extract DNA from 2000 GM20431 cells *Elute DNA with 8uL elution buffer *Use all 8uL dna as template for one pair of prim...)
 
>Alan6017518
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*Perform serial dilution to obtain
*Perform serial dilution to obtain
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''200ng'''
| align="center" style="background:#f0f0f0;"|200ng methylated DNA
|-
| 100ng methylated DNA
| 100ng methylated DNA
|-
|-

Revision as of 19:25, 14 August 2009

EZ DNA Methylation Direct - GM20431 cells

Objective

  • Extract DNA from 2000 GM20431 cells
  • Elute DNA with 8uL elution buffer
  • Use all 8uL dna as template for one pair of primer
  • Quantify amount of DNA with Nanodrop Qubit and Gel quantification
  • Perform serial dilution to obtain
200ng methylated DNA 100ng methylated DNA
50ng methylated DNA
30ng methylated DNA
10ng methylated DNA