AlanFung:LabNotes/EZ/2009-8-14: Difference between revisions

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>Alan6017518
>Alan6017518
 
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| 10ng methylated DNA
| 10ng methylated DNA
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{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''2000 cells'''
| align="center" style="background:#f0f0f0;"|'''100,000 cells'''
|-
| M-Digestion Buffer||10ul||13ul
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| Sample||up to 9ul||up to 12ul
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| Proteinase K||1ul||1ul
|-
| H20||X ul||x ul
|-
| Total Volume||20ul||26 ul
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|  
|  
|}
|}

Latest revision as of 20:32, 14 August 2009

EZ DNA Methylation Direct - GM20431 cells[edit]

Objective[edit]

  • Extract DNA from 2000 GM20431 cells
  • Elute DNA with 8uL elution buffer
  • Use all 8uL dna as template for one pair of primer
  • Quantify amount of DNA with Nanodrop Qubit and Gel quantification
  • Perform serial dilution to obtain
200ng methylated DNA
100ng methylated DNA
50ng methylated DNA
30ng methylated DNA
10ng methylated DNA
' 2000 cells 100,000 cells
M-Digestion Buffer 10ul 13ul
Sample up to 9ul up to 12ul
Proteinase K 1ul 1ul
H20 X ul x ul
Total Volume 20ul 26 ul