AlanFung:LabNotes/EZ/2009-8-14: Difference between revisions
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| 10ng methylated DNA | | 10ng methylated DNA | ||
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{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''2000 cells''' | |||
| align="center" style="background:#f0f0f0;"|'''100,000 cells''' | |||
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| M-Digestion Buffer||10ul||13ul | |||
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| Sample||up to 9ul||up to 12ul | |||
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| Proteinase K||1ul||1ul | |||
|- | |||
| H20||X ul||x ul | |||
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| Total Volume||20ul||26 ul | |||
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|} | |} |
Latest revision as of 20:32, 14 August 2009
EZ DNA Methylation Direct - GM20431 cells[edit]
Objective[edit]
- Extract DNA from 2000 GM20431 cells
- Elute DNA with 8uL elution buffer
- Use all 8uL dna as template for one pair of primer
- Quantify amount of DNA with Nanodrop Qubit and Gel quantification
- Perform serial dilution to obtain
200ng methylated DNA |
100ng methylated DNA |
50ng methylated DNA |
30ng methylated DNA |
10ng methylated DNA |
' | 2000 cells | 100,000 cells |
M-Digestion Buffer | 10ul | 13ul |
Sample | up to 9ul | up to 12ul |
Proteinase K | 1ul | 1ul |
H20 | X ul | x ul |
Total Volume | 20ul | 26 ul |