AlanFung:LabNotes/DNA/2009-8-18: Difference between revisions

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>Alan6017518
(New page: ==Digestion with MmeI== No_1_2_3_9 x4 Total 30ul dUTP_PCR 20ul ...)
 
>Alan6017518
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==Digestion with MmeI==
==Digestion with MmeI==
                                      No_1_2_3_9    x4
{| {{table}}
  Total                                30ul                 
| align="center" style="background:#f0f0f0;"|''''''
  dUTP_PCR                            20ul       
| align="center" style="background:#f0f0f0;"|'''PGP4'''
  10X NEBuffer 4                       4ul          16         
| align="center" style="background:#f0f0f0;"|'''PGP6'''
  1mM SAM(fresh)                       4ul          16           
| align="center" style="background:#f0f0f0;"|'''PGP10'''
  2U/ul Mme I                          8ul          32             
| align="center" style="background:#f0f0f0;"|'''A'''
  ddH2O                                4ul          16
| align="center" style="background:#f0f0f0;"|'''B'''
|-
| Sample||20||20||20||10||10
|-
| 10X NEBuffer 4||4||4||4||3||3
|-
| 1mM SAM (fresh)||4||4||4||3||3
|-
| 2U/ul MmeI||1||1||1||1||1
|-
| ddH20||11||11||11||13||13
|-
| Total||40||40||40||30||30
|-
|
|}
    
    
   1mM SAM: 32mM SAM 1ul + 31ul ddH2O.
   1mM SAM: 32mM SAM 1ul + 31ul ddH2O.
   37C 2h. MinEulte column purification. Elute in 12ul EB.
   37C 2h. MinEulte column purification. Elute in 12ul EB.

Revision as of 19:19, 18 August 2009

Digestion with MmeI

' PGP4 PGP6 PGP10 A B
Sample 20 20 20 10 10
10X NEBuffer 4 4 4 4 3 3
1mM SAM (fresh) 4 4 4 3 3
2U/ul MmeI 1 1 1 1 1
ddH20 11 11 11 13 13
Total 40 40 40 30 30
 1mM SAM: 32mM SAM 1ul + 31ul ddH2O.
 37C 2h. MinEulte column purification. Elute in 12ul EB.