AlanFung:LabNotes/DNA/2009-8-18: Difference between revisions
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>Alan6017518 (New page: ==Digestion with MmeI== No_1_2_3_9 x4 Total 30ul dUTP_PCR 20ul ...) |
>Alan6017518 |
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==Digestion with MmeI== | ==Digestion with MmeI== | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''PGP4''' | |||
| align="center" style="background:#f0f0f0;"|'''PGP6''' | |||
| align="center" style="background:#f0f0f0;"|'''PGP10''' | |||
| align="center" style="background:#f0f0f0;"|'''A''' | |||
| align="center" style="background:#f0f0f0;"|'''B''' | |||
|- | |||
| Sample||20||20||20||10||10 | |||
|- | |||
| 10X NEBuffer 4||4||4||4||3||3 | |||
|- | |||
| 1mM SAM (fresh)||4||4||4||3||3 | |||
|- | |||
| 2U/ul MmeI||1||1||1||1||1 | |||
|- | |||
| ddH20||11||11||11||13||13 | |||
|- | |||
| Total||40||40||40||30||30 | |||
|- | |||
| | |||
|} | |||
1mM SAM: 32mM SAM 1ul + 31ul ddH2O. | 1mM SAM: 32mM SAM 1ul + 31ul ddH2O. | ||
37C 2h. MinEulte column purification. Elute in 12ul EB. | 37C 2h. MinEulte column purification. Elute in 12ul EB. |
Revision as of 19:19, 18 August 2009
Digestion with MmeI
' | PGP4 | PGP6 | PGP10 | A | B |
Sample | 20 | 20 | 20 | 10 | 10 |
10X NEBuffer 4 | 4 | 4 | 4 | 3 | 3 |
1mM SAM (fresh) | 4 | 4 | 4 | 3 | 3 |
2U/ul MmeI | 1 | 1 | 1 | 1 | 1 |
ddH20 | 11 | 11 | 11 | 13 | 13 |
Total | 40 | 40 | 40 | 30 | 30 |
1mM SAM: 32mM SAM 1ul + 31ul ddH2O. 37C 2h. MinEulte column purification. Elute in 12ul EB.