AlanFung:LabNotes/DNA/2009-8-18: Difference between revisions
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==Digestion with MmeI== | ==Digestion with MmeI== | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"| | | align="center" style="background:#f0f0f0;"| | ||
| align="center" style="background:#f0f0f0;"|'''PGP4''' | | align="center" style="background:#f0f0f0;"|'''PGP4''' | ||
| align="center" style="background:#f0f0f0;"|'''PGP6''' | | align="center" style="background:#f0f0f0;"|'''PGP6''' |
Revision as of 19:21, 18 August 2009
Digestion with MmeI
PGP4 | PGP6 | PGP10 | A | B | |
Sample | 20 | 20 | 20 | 10 | 10 |
10X NEBuffer 4 | 4 | 4 | 4 | 3 | 3 |
1mM SAM (fresh) | 4 | 4 | 4 | 3 | 3 |
2U/ul MmeI | 1 | 1 | 1 | 1 | 1 |
ddH20 | 11 | 11 | 11 | 13 | 13 |
Total | 40 | 40 | 40 | 30 | 30 |
1mM SAM: 32mM SAM 1ul + 31ul ddH2O. 37C 2h.
- Qiaquick column purification. Elute in 12ul EB.