AlanFung:LabNotes/DNA/2009-8-18: Difference between revisions

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==Digestion with MmeI==
==Digestion with MmeI==
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|
| align="center" style="background:#f0f0f0;"|'''PGP4'''
| align="center" style="background:#f0f0f0;"|'''PGP4'''
| align="center" style="background:#f0f0f0;"|'''PGP6'''
| align="center" style="background:#f0f0f0;"|'''PGP6'''

Revision as of 19:21, 18 August 2009

Digestion with MmeI

PGP4 PGP6 PGP10 A B
Sample 20 20 20 10 10
10X NEBuffer 4 4 4 4 3 3
1mM SAM (fresh) 4 4 4 3 3
2U/ul MmeI 1 1 1 1 1
ddH20 11 11 11 13 13
Total 40 40 40 30 30
 1mM SAM: 32mM SAM 1ul + 31ul ddH2O.
 37C 2h. 
  • Qiaquick column purification. Elute in 12ul EB.