Alice:LabNotes/2009-9-7: Difference between revisions
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>Zsakura2 (New page: ==Re-amplification of PhiX control library== PCR: x4 PhiX control DNA(10nM) 0.025ul 0.1ul Syb_FP5(100uM) 0.2ul ...) |
>Zsakura2 |
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Line 8: | Line 8: | ||
50x SYBR geen 0.2ul 0.8ul | 50x SYBR geen 0.2ul 0.8ul | ||
H2O 50ul 200ul | H2O 50ul 200ul | ||
98c 30sec -> (98C 10sec -> 65C 20sec -> 72C 20sec) X 11 cycles -> 72C 3min -> 15C | 98c 30sec -> (98C 10sec -> 65C 20sec -> 72C 20sec) X 11 cycles -> 72C 3min -> 15C | ||
Qiaquick column purification and elute in 30ul of EB. | Qiaquick column purification and elute in 30ul of EB. | ||
QPCR result: 1.59nM | QPCR result: 1.59nM |
Revision as of 23:14, 14 September 2009
Re-amplification of PhiX control library
PCR: x4 PhiX control DNA(10nM) 0.025ul 0.1ul Syb_FP5(100uM) 0.2ul 0.8ul Syb_RP7(100uM) 0.2ul 0.8ul 2x phusion master mix 50ul 200ul 50x SYBR geen 0.2ul 0.8ul H2O 50ul 200ul 98c 30sec -> (98C 10sec -> 65C 20sec -> 72C 20sec) X 11 cycles -> 72C 3min -> 15C
Qiaquick column purification and elute in 30ul of EB. QPCR result: 1.59nM