AlanFung:LabNotes/Cell/2009-10-10: Difference between revisions
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==MEF== | |||
*Make 9x10cm MEF plates for MCM | |||
==HUES9== | |||
Split (1:8)from 1x10cm to 1 well (MEF) and 1x10cm (Matrigel) | |||
==H9== | |||
Split (1:6) form 1x6well to 1x10cm (Matrigel) and (1:6) 1x6well to 3x6wells | |||
==HUES53== | |||
*Check and Feed, Images below | |||
*Split 1:2 10cm matrigel dishes when confluent, then collect the day after. | |||
==MEF== | ==MEF== | ||
*Backup plates looks really good | *Backup plates looks really good |
Latest revision as of 02:17, 11 October 2009
MEF[edit]
- Make 9x10cm MEF plates for MCM
HUES9[edit]
Split (1:8)from 1x10cm to 1 well (MEF) and 1x10cm (Matrigel)
H9[edit]
Split (1:6) form 1x6well to 1x10cm (Matrigel) and (1:6) 1x6well to 3x6wells
HUES53[edit]
- Check and Feed, Images below
- Split 1:2 10cm matrigel dishes when confluent, then collect the day after.
MEF[edit]
Matrigel[edit]
- HUES 53 appears to form more elongated colonies with sharper edges, no signs of differentiation.
- 1:5