AlanFung:LabNotes/Cell/2009-10-11: Difference between revisions
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>Alan6017518 (→H9) |
>Alan6017518 (→HUES9) |
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==HUES9== | ==HUES9== | ||
*Feed 1x10cm (matrigel) + 1 well (mef) w/ 20% HUES MCM | *Feed 1x10cm (matrigel) + 1 well (mef) w/ 20% HUES MCM | ||
*[[Media:SNAP-201933-0002.JPG]] | |||
==H9== | ==H9== |
Latest revision as of 18:18, 12 October 2009
MEF[edit]
- Make 3x10cm MEF plates for HUES MCM
- Make 3x10cm MEF plates for Wicell MCM
HUES9[edit]
- Feed 1x10cm (matrigel) + 1 well (mef) w/ 20% HUES MCM
- Media:SNAP-201933-0002.JPG
H9[edit]
- Feed 1x10cm (matrigel) + 3 x 6 wells (mef) w/ 100% wicell mcm
- Media:SNAP-202118-0003.JPG
HUES53[edit]
- Cells are ~80% confluent, split 1x10cm (matrigel) (1:2) to 2x10cm (matrigel) w/100% HUES MCM
- Feed 3 wells (mef) w/ 20% HUES MCM
- Cells are probably ready to collect on Monday
Matrigel[edit]
- HUES 53 appears to form more elongated colonies with sharper edges, no signs of differentiation.
- Media:SNAP-201653-0001.jpg