AlanFung:LabNotes/Sequencing/2009-11-18: Difference between revisions
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>Alan6017518 (New page: ==Re-amplification of PhiX control library== PCR: x4 PhiX control DNA(10nM) 0.025ul 0.1ul Syb_FP5(100uM) 0.2ul ...) |
>Alan6017518 |
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Line 9: | Line 9: | ||
H2O 50ul 200ul | H2O 50ul 200ul | ||
98c 30sec -> (98C 10sec -> 65C 20sec -> 72C 20sec) X | 98c 30sec -> (98C 10sec -> 65C 20sec -> 72C 20sec) X 5 cycles -> 72C 3min -> 15C | ||
Qiaquick column purification and elute in 30ul of EB. | Qiaquick column purification and elute in 30ul of EB. | ||
Nanodrop result: | |||
PAGE gel result: | PAGE gel result: | ||
QPCR result: | QPCR result: |
Revision as of 01:24, 19 November 2009
Re-amplification of PhiX control library
PCR: x4 PhiX control DNA(10nM) 0.025ul 0.1ul Syb_FP5(100uM) 0.2ul 0.8ul Syb_RP7(100uM) 0.2ul 0.8ul 2x phusion master mix 50ul 200ul 50x SYBR geen 0.2ul 0.8ul H2O 50ul 200ul 98c 30sec -> (98C 10sec -> 65C 20sec -> 72C 20sec) X 5 cycles -> 72C 3min -> 15C
Qiaquick column purification and elute in 30ul of EB.
Nanodrop result:
PAGE gel result: QPCR result: