AlanFung:LabNotes/Sequencing/2009-11-19: Difference between revisions
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>Alan6017518 |
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| PhiX-Stock||CVF||PGP4||PGP10||PGP8C | | PhiX-Stock||CVF||PGP4||PGP10||PGP8C | ||
|- | |- | ||
| PhiX||1-7 PGP1||PGP6||NA12878||PGP8D | | PhiX-Stock||1-7 PGP1||PGP6||NA12878||PGP8D | ||
|- | |- | ||
| PhiX||1-7 PGP1||PGP6||NA12878||PGP8D | | PhiX-Stock||1-7 PGP1||PGP6||NA12878||PGP8D | ||
|- | |- | ||
| PhiX||8 PGP1||PGP8||PGP8A||PGP8E | | PhiX||8 PGP1||PGP8||PGP8A||PGP8E |
Revision as of 18:38, 19 November 2009
Quantification of New amplified Phi-X and all libraries
- Dilute all samples by 10 fold
Samples | ||||
PhiX-Stock | CVF | PGP4 | PGP10 | PGP8C |
PhiX-Stock | CVF | PGP4 | PGP10 | PGP8C |
PhiX-Stock | 1-7 PGP1 | PGP6 | NA12878 | PGP8D |
PhiX-Stock | 1-7 PGP1 | PGP6 | NA12878 | PGP8D |
PhiX | 8 PGP1 | PGP8 | PGP8A | PGP8E |
PhiX | 8 PGP1 | PGP8 | PGP8A | PGP8E |
CVI | 9 PGP1 | PGP9 | PGP8B | N12 |
CVI | 9 PGP1 | PGP9 | PGP8B | N12 |
- split every 50ul rxn into 2 wells
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
X20 | X1.1 | ||
PhiX Control DNA (10nM) | 1.000 | ||
Syb_FP5 (100uM) | 0.200 | 4.000 | 4.400 |
Syb_RP7 (100uM) | 0.200 | 4.000 | 4.400 |
2X Phusion | 25.000 | 500.000 | 550.000 |
50X SYBR Green I | 0.200 | 4.000 | 4.400 |
H20 | 24.000 | 480.000 | 528.000 |