AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions

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>Alan6017518
(New page: ==CVF-N== *Reamplify library and ship it out for shearing {| {{table}} | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' | align="cent...)
 
>Alan6017518
Line 8: Line 8:
| align="center" style="background:#f0f0f0;"|'''X1.1'''
| align="center" style="background:#f0f0f0;"|'''X1.1'''
|-
|-
| Library||12||0.25||1||1.1
| Library||1||-||-||-
|
| 2X Phusion||50||50||750||825
|-
|-
| 2X Phusion||50||50||200||220
| AmpF6.3NH2 (100uM)||2||2||3||3.3
|-
|-
| AmpF6.3NH2 (10uM)||2||2||8||8.8
| AmpR6.3NH2 (100uM)||2||2||3||3.3
|-
|-
| AmpR6.3NH2 (10uM)||2||2||8||8.8
| 50X SYBR Green I||2||2||3||3.3
|-
|-
| 10X SYBR Green I||2||2||8||8.8
| dH2O||32||43.75||645||709.5
|-
| dH2O||32||43.75||175||192.5
|-
|-
|  
|  

Revision as of 05:34, 23 November 2009

CVF-N

  • Reamplify library and ship it out for shearing
' ' ' x4 X1.1
Library 1 - - - 2X Phusion 50 50 750 825
AmpF6.3NH2 (100uM) 2 2 3 3.3
AmpR6.3NH2 (100uM) 2 2 3 3.3
50X SYBR Green I 2 2 3 3.3
dH2O 32 43.75 645 709.5
  • 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min