AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions
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>Alan6017518 (New page: ==CVF-N== *Reamplify library and ship it out for shearing {| {{table}} | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' | align="cent...) |
>Alan6017518 (→CVF-N) |
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| align="center" style="background:#f0f0f0;"|'''X1.1''' | | align="center" style="background:#f0f0f0;"|'''X1.1''' | ||
|- | |- | ||
| Library|| | | Library||1||-||-||- | ||
| | |||
| 2X Phusion||50||50||750||825 | |||
|- | |- | ||
| | | AmpF6.3NH2 (100uM)||2||2||3||3.3 | ||
|- | |- | ||
| | | AmpR6.3NH2 (100uM)||2||2||3||3.3 | ||
|- | |- | ||
| | | 50X SYBR Green I||2||2||3||3.3 | ||
|- | |- | ||
| dH2O||32||43.75||645||709.5 | |||
| dH2O||32||43.75|| | |||
|- | |- | ||
| | | |
Revision as of 05:34, 23 November 2009
CVF-N
- Reamplify library and ship it out for shearing
' | ' | ' | x4 | X1.1 | ||||||
Library | 1 | - | - | - | 2X Phusion | 50 | 50 | 750 | 825 | |
AmpF6.3NH2 (100uM) | 2 | 2 | 3 | 3.3 | ||||||
AmpR6.3NH2 (100uM) | 2 | 2 | 3 | 3.3 | ||||||
50X SYBR Green I | 2 | 2 | 3 | 3.3 | ||||||
dH2O | 32 | 43.75 | 645 | 709.5 | ||||||
- 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min