AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions

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>Alan6017518
>Alan6017518
Line 6: Line 6:
| align="center" style="background:#f0f0f0;"|'''x15'''
| align="center" style="background:#f0f0f0;"|'''x15'''
| align="center" style="background:#f0f0f0;"|'''X1.1'''
| align="center" style="background:#f0f0f0;"|'''X1.1'''
| align="center" style="background:#f0f0f0;"|'''/2'''
|-
|-
| Library||1||-||-||
| Library||1||-||-||-||
|-
|-
| 2X Phusion||50||750||825
| 2X Phusion||50||750||825||411.5
|-
|-
| AmpF6.3NH2 (100uM)||0.2||3||3.3
| AmpF6.3NH2 (100uM)||0.2||3||3.3||1.65
|-
|-
| AmpR6.3NH2 (100uM)||0.2||3||3.3
| AmpR6.3NH2 (100uM)||0.2||3||3.3||1.65
|-
|-
| 50X SYBR Green I||0.2||3||3.3
| 50X SYBR Green I||0.2||3||3.3||1.65
|-
|-
| dH2O||43||645||709.5
| dH2O||43||645||709.5||354.75
|-
|-
|  
|  

Revision as of 05:38, 23 November 2009

CVF-N

  • Reamplify library and ship it out for shearing
' ' x15 X1.1 /2
Library 1 - - -
2X Phusion 50 750 825 411.5
AmpF6.3NH2 (100uM) 0.2 3 3.3 1.65
AmpR6.3NH2 (100uM) 0.2 3 3.3 1.65
50X SYBR Green I 0.2 3 3.3 1.65
dH2O 43 645 709.5 354.75
  • 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min