AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions

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>Alan6017518
>Alan6017518
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| align="center" style="background:#f0f0f0;"|'''/2'''
| align="center" style="background:#f0f0f0;"|'''/2'''
|-
|-
| Library||1||-||-||-||
| Library||1||-||-||7.66||
|-
|-
| 2X Phusion||50||750||825||412.5
| 2X Phusion||50||750||825||412.5

Revision as of 05:59, 23 November 2009

CVF-N

  • Reamplify library and ship it out for shearing
' ' x15 X1.1 /2
Library 1 - - 7.66
2X Phusion 50 750 825 412.5
AmpF6.3NH2 (100uM) 0.2 3 3.3 1.65
AmpR6.3NH2 (100uM) 0.2 3 3.3 1.65
50X SYBR Green I 0.2 3 3.3 1.65
dH2O 43 645 709.5 354.75
  • 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min