AlanFung:Protocol/Construction of Solexa sequencing Library(USER): Difference between revisions

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>Alan6017518
(New page: ==Sequencing Library Construction with Single Adaptor Protocol== ===PCR with dUTP and 6.3NH2 Primer=== {| {{table}} | ||1 well |- | H2O||47.4 |- | Amp F6.3NH2(100uM)||0.2 |- | Amp R6.3NH2 ...)
 
>Alan6017518
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==Sequencing Library Construction with Single Adaptor Protocol==
==Sequencing Library Construction with Single Adaptor Protocol==
===PCR with dUTP and 6.3NH2 Primer===
==PCR with dUTP and 6.3NH2 Primer==
{| {{table}}
{| {{table}}
| ||1 well
| ||1 well

Revision as of 16:36, 24 November 2009

Sequencing Library Construction with Single Adaptor Protocol

PCR with dUTP and 6.3NH2 Primer

1 well
H2O 47.4
Amp F6.3NH2(100uM) 0.2
Amp R6.3NH2 (100uM) 0.2
dUTP (1mM) 0.8
50X SYBG I 0.4
2X Master Mix 50
Template 1
Total 100