Dinh:Protocols/Probes Prep Nov24: Difference between revisions
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>Dinh m (New page: KAPA SYBR FAST qPCR Kit Master Mix (2x) Universal ideally suited for gene expression analysis low copy gene detection microarray validation gene knockdown validation Notes: protect k...) |
>Dinh mNo edit summary |
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always ensure that the product has been fully thawed and mixed before use | always ensure that the product has been fully thawed and mixed before use | ||
do not exceed 25 ul reaction volumes | do not exceed 25 ul reaction volumes | ||
do not exceed 20ng template DNA per 20 ul reaction volume | |||
novel enzyme in kit enhances the amplification efficiency of both high GC and AT templates | novel enzyme in kit enhances the amplification efficiency of both high GC and AT templates | ||
Chromo4 RT Detector does not require ROX Reference dye |
Revision as of 22:02, 24 November 2009
KAPA SYBR FAST qPCR Kit Master Mix (2x) Universal
ideally suited for gene expression analysis low copy gene detection microarray validation gene knockdown validation
Notes:
protect kit components from light always ensure that the product has been fully thawed and mixed before use do not exceed 25 ul reaction volumes do not exceed 20ng template DNA per 20 ul reaction volume novel enzyme in kit enhances the amplification efficiency of both high GC and AT templates Chromo4 RT Detector does not require ROX Reference dye