Dinh:LAB/Bisulfite Sequencing: Difference between revisions

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*[[Dinh:Protocols/Probes_Prep_Nov24| Nov 24 2009 Probes Preparation]], CpG30K, KAPA qPCR kit from KAPA Biosystems, Nanodrop: 21.4 ug, End point [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-4 QUBIT]: 23ng/ul x 20 ul
*[[Dinh:Protocols/Probes_Prep_Nov24| Nov 24 2009 Probes Preparation]], CpG30K, KAPA qPCR kit from KAPA Biosystems, Nanodrop: 21.4 ug, End point [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-4 QUBIT]: 23ng/ul x 20 ul


*[http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-29 Dec 30 Probes Set] 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).
*[http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-29 Dec 30 CpG30K Probes Set] 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).


=DNA Extraction=
=DNA Extraction=

Revision as of 20:29, 23 January 2010

Probes Preparation

DNA Extraction

Bisulfite Conversion and Capturing

  • Improving capturing efficiency of CpG30K set for pipelining, Jan 4 2010 Capture Reaction (2pm), Jan 7 2010 Results.

Breast Cancer Patients, Diet and Methylation Study

  • Capturing Breast Cancer Patients (samples 1-9), Dec 9 2009 Capture Reaction, Dec 15 2009 E-Gel Size selection, Amplification with iProof
Breast Cancer Patients (1-28) Samples Track, Dinh:Breast_Cancer_Methylation
  • Capturing Test, CpG97A/B. Templates: bis_Jurkat, bis_16. Conditions: 55C vs 60C, 0 Cycling. Jan 10 2010
  • Capture, CpG97A/B. Templates: bis_10-14, bis_Jurkat. Condition: 55C, 0 Cycling. Jan 15 2010
  • Capture Re-test for Sanger Sequencing, CpG97A/B. Template: bis_15. Condition: 55C vs 60C, 0 Cycling. Jan 21 2010

Notebook

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2010/07/01 view=oneyear </calendar>

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2009/07/01 view=oneyear </calendar>