AlanFung:LabNotes/Sequencing/2009-11-27: Difference between revisions

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*Ethanol Precipitation
*Ethanol Precipitation
*Dissolve all in 10ul ddH2O
*Dissolve all in 14ul ddH2O
==QPCR Quantification==


Setup
Stock Phi-X (1nM)  CVF-N (Shearing) (1/10)
Stock Phi-X (1nM)  CVF-N (Shearing) (1/10)
Stock Phi-X (1nM)  CVF-N (Shearing) (1/10)
Stock Phi-X (1nM)  CVF-N (Shearing) (1/10)
Amp Phi-X (1nM)    CVF-N (A/B) (1/10)
Amp Phi-X (1nM)    CVF-N (A/B) (1/10)
Amp Phi-X (1nM)    CVF-N (A/B) (1/10)
Amp Phi-X (1nM)    CVF-N (A/B) (1/10)
{| {{table}}
{| {{table}}
| ||||X8||X1.1
| ||||X8||X1.1

Revision as of 06:35, 27 November 2009

  • Ethanol Precipitation
  • Dissolve all in 14ul ddH2O

QPCR Quantification

Setup
Stock Phi-X (1nM)   CVF-N (Shearing) (1/10)
Stock Phi-X (1nM)   CVF-N (Shearing) (1/10)
Stock Phi-X (1nM)   CVF-N (Shearing) (1/10)
Stock Phi-X (1nM)   CVF-N (Shearing) (1/10)
Amp Phi-X (1nM)     CVF-N (A/B) (1/10)
Amp Phi-X (1nM)     CVF-N (A/B) (1/10)
Amp Phi-X (1nM)     CVF-N (A/B) (1/10)
Amp Phi-X (1nM)     CVF-N (A/B) (1/10)
X8 X1.1
DNA 1.000
Syb_FP5 (100uM) 0.200 1.600 1.760
Syb_RP7 (100uM) 0.200 1.600 1.760
2X Phusion 25.000 200.000 220.000
50X SYBR Green I 0.200 1.600 1.760
dH2O 24.000 192.000 211.200