AlanFung:LabNotes/Sequencing/2009-11-27: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 No edit summary |
>Alan6017518 No edit summary |
||
Line 1: | Line 1: | ||
*Ethanol Precipitation | *Ethanol Precipitation | ||
*Dissolve all in | *Dissolve all in 14ul ddH2O | ||
==QPCR Quantification== | |||
Setup | |||
Stock Phi-X (1nM) CVF-N (Shearing) (1/10) | |||
Stock Phi-X (1nM) CVF-N (Shearing) (1/10) | |||
Stock Phi-X (1nM) CVF-N (Shearing) (1/10) | |||
Stock Phi-X (1nM) CVF-N (Shearing) (1/10) | |||
Amp Phi-X (1nM) CVF-N (A/B) (1/10) | |||
Amp Phi-X (1nM) CVF-N (A/B) (1/10) | |||
Amp Phi-X (1nM) CVF-N (A/B) (1/10) | |||
Amp Phi-X (1nM) CVF-N (A/B) (1/10) | |||
{| {{table}} | {| {{table}} | ||
| ||||X8||X1.1 | | ||||X8||X1.1 |
Revision as of 06:35, 27 November 2009
- Ethanol Precipitation
- Dissolve all in 14ul ddH2O
QPCR Quantification
Setup Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10)
X8 | X1.1 | ||
DNA | 1.000 | ||
Syb_FP5 (100uM) | 0.200 | 1.600 | 1.760 |
Syb_RP7 (100uM) | 0.200 | 1.600 | 1.760 |
2X Phusion | 25.000 | 200.000 | 220.000 |
50X SYBR Green I | 0.200 | 1.600 | 1.760 |
dH2O | 24.000 | 192.000 | 211.200 |