AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 4: Difference between revisions

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*Mix 50/50 old/new Jurkat to make up 10ng
New: 0.905ul
Old: 0.48ul

Revision as of 18:24, 4 December 2009

qPCR Validation(3 Bisulfite Conversion Primers + gDNA primers)

  • Start with 10ng template
Sample Sample Concentration ng/ul To get 10ng 1/10 Dilution ng/ul To get 10ng
NA12878 17.6 0.57
NA18507 12.4 0.81
NA20431 50.6 5.06 1.98
CV-Fibr 4.84 2.07
CV-iPS-B 7.27 1.38
CV-iPS-F 6.91 1.45
Jurkat 55.4 5.54 1.81
Jurkat (old) 104 10.4 0.96
  • Mix 50/50 old/new Jurkat to make up 10ng
New: 0.905ul
Old: 0.48ul