AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 4: Difference between revisions
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>Alan6017518 |
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Line 25: | Line 25: | ||
New: 0.905ul | New: 0.905ul | ||
Old: 0.48ul | Old: 0.48ul | ||
{| {{table}} | |||
| Sample||Volume||Make it up to 8ul | |||
|- | |||
| NA12878||0.57||7.43 | |||
|- | |||
| NA18507||0.81||7.19 | |||
|- | |||
| NA20431||1.98||6.02 | |||
|- | |||
| CV-Fibr||2.07||5.93 | |||
|- | |||
| CV-iPS-B||1.38||6.62 | |||
|- | |||
| CV-iPS-F||1.45||6.55 | |||
|- | |||
| Jurkat||1.81||6.19 | |||
|- | |||
| Jurkat (old)||0.96||7.04 | |||
|- | |||
| Jurkat (mix)||1.39||6.62 | |||
|- | |||
| | |||
|} |
Revision as of 18:33, 4 December 2009
qPCR Validation(3 Bisulfite Conversion Primers + gDNA primers)
- Start with 10ng template
Sample | Sample Concentration ng/ul | To get 10ng | 1/10 Dilution ng/ul | To get 10ng |
NA12878 | 17.6 | 0.57 | ||
NA18507 | 12.4 | 0.81 | ||
NA20431 | 50.6 | 5.06 | 1.98 | |
CV-Fibr | 4.84 | 2.07 | ||
CV-iPS-B | 7.27 | 1.38 | ||
CV-iPS-F | 6.91 | 1.45 | ||
Jurkat | 55.4 | 5.54 | 1.81 | |
Jurkat (old) | 104 | 10.4 | 0.96 | |
- Mix 50/50 old/new Jurkat to make up 10ng
New: 0.905ul Old: 0.48ul
Sample | Volume | Make it up to 8ul |
NA12878 | 0.57 | 7.43 |
NA18507 | 0.81 | 7.19 |
NA20431 | 1.98 | 6.02 |
CV-Fibr | 2.07 | 5.93 |
CV-iPS-B | 1.38 | 6.62 |
CV-iPS-F | 1.45 | 6.55 |
Jurkat | 1.81 | 6.19 |
Jurkat (old) | 0.96 | 7.04 |
Jurkat (mix) | 1.39 | 6.62 |