AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 4: Difference between revisions

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>Alan6017518
>Alan6017518
Line 25: Line 25:
  New: 0.905ul
  New: 0.905ul
  Old: 0.48ul
  Old: 0.48ul
{| {{table}}
| Sample||Volume||Make it up to 8ul
|-
| NA12878||0.57||7.43
|-
| NA18507||0.81||7.19
|-
| NA20431||1.98||6.02
|-
| CV-Fibr||2.07||5.93
|-
| CV-iPS-B||1.38||6.62
|-
| CV-iPS-F||1.45||6.55
|-
| Jurkat||1.81||6.19
|-
| Jurkat (old)||0.96||7.04
|-
| Jurkat (mix)||1.39||6.62
|-
|
|}

Revision as of 18:33, 4 December 2009

qPCR Validation(3 Bisulfite Conversion Primers + gDNA primers)

  • Start with 10ng template
Sample Sample Concentration ng/ul To get 10ng 1/10 Dilution ng/ul To get 10ng
NA12878 17.6 0.57
NA18507 12.4 0.81
NA20431 50.6 5.06 1.98
CV-Fibr 4.84 2.07
CV-iPS-B 7.27 1.38
CV-iPS-F 6.91 1.45
Jurkat 55.4 5.54 1.81
Jurkat (old) 104 10.4 0.96
  • Mix 50/50 old/new Jurkat to make up 10ng
New: 0.905ul
Old: 0.48ul
Sample Volume Make it up to 8ul
NA12878 0.57 7.43
NA18507 0.81 7.19
NA20431 1.98 6.02
CV-Fibr 2.07 5.93
CV-iPS-B 1.38 6.62
CV-iPS-F 1.45 6.55
Jurkat 1.81 6.19
Jurkat (old) 0.96 7.04
Jurkat (mix) 1.39 6.62