AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 10: Difference between revisions
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==TBE Gel Quantification== |
Revision as of 21:10, 10 December 2009
PCR Amplification of Captured product
1well (ul) X6 Template 10 2X iProof Mastermix 50 300 AmpF6.2SoL (10uM) 4 24 AmpR6.2Sol (10uM) 4 24 50X SYBG I 0.8 4.8 H2O 31.2 187.2 Total Volume 90.000
98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold
Setup NA12878 NA18507 NA20431 Jurkat New Jurkat Old
Setup |
NA12878 |
NA18507 |
NA20431 |
Jurkat New |
Jurkat Old |