AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 10: Difference between revisions

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==TBE Gel Quantification==

Revision as of 21:10, 10 December 2009

PCR Amplification of Captured product

1well (ul) X6 Template 10 2X iProof Mastermix 50 300 AmpF6.2SoL (10uM) 4 24 AmpR6.2Sol (10uM) 4 24 50X SYBG I 0.8 4.8 H2O 31.2 187.2 Total Volume 90.000

98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold

Setup NA12878 NA18507 NA20431 Jurkat New Jurkat Old

Setup
NA12878
NA18507
NA20431
Jurkat New
Jurkat Old

TBE Gel Quantification