AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 10: Difference between revisions

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>Alan6017518
>Alan6017518
Line 7: Line 7:
| 2X iProof Mastermix||50||300
| 2X iProof Mastermix||50||300
|-
|-
| AmpF6.2SoL (10uM)||4||24
| AmpF6.2SoL (10u0M)||0.4||2.4
|-
|-
| AmpR6.2Sol (10uM)||4||24
| AmpR6.2Sol (100uM)||0.4||2.4
|-
|-
| 50X SYBG I||0.8||4.8
| 50X SYBG I||0.8||4.8

Revision as of 23:24, 10 December 2009

PCR Amplification of Captured product

1well (ul) X6
Template 10
2X iProof Mastermix 50 300
AmpF6.2SoL (10u0M) 0.4 2.4
AmpR6.2Sol (100uM) 0.4 2.4
50X SYBG I 0.8 4.8
H2O 31.2 187.2
Total Volume 90.000

98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold

Setup
NA12878
NA18507
NA20431
Jurkat New
Jurkat Old

TBE Gel Quantification