AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 31: Difference between revisions

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>Alan6017518
(New page: ==PCR Amplification of Captured product== {| {{table}} | ||1well (ul)||X6 |- | Template||10|| |- | 2X iProof Mastermix||50||300 |- | AmpF6.2SoL (10u0M)||0.4||2.4 |- | AmpR6.2Sol (100uM)||0...)
 
>Alan6017518
Line 1: Line 1:
==PCR Amplification of Captured product==
==PCR Amplification of Captured product==
{| {{table}}
{| {{table}}
| ||1well (ul)||X6
| ||1 well (uL)||X 6||||||1 well (uL)||X 6
|-
|-
| Template||10||
| Template||10||||||Template||||
|-
|-
| 2X iProof Mastermix||50||300
| 2X iProof Mastermix||50||300||||2X iProof Mastermix||50||300
|-
|-
| AmpF6.2SoL (10u0M)||0.4||2.4
| Amp F6.3 Sol (100uM)||0.4||2.4||||Amp F6.3r Sol (100uM)||0.4||2.4
|-
|-
| AmpR6.2Sol (100uM)||0.4||2.4
| Amp R6.3Sol (100uM)||0.4||2.4||||Amp R6.3r Sol (100uM)||0.4||2.4
|-
|-
| 50X SYBG I||0.8||4.8
| 50X SYBG I||0.8||4.8||||50X SYBG I||0.8||4.8
|-
|-
| H2O||31.2||187.2
| H2O||31.2||187.2||||H2O||31.2||187.2
|-
|-
| Total Volume||90.000||
| Total Volume||90||540||||Total Volume||90||540
|-
|-
|  
|  
|}
|}


98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold
98C 30S -> (98C 10S -> 60C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold

Revision as of 21:14, 31 December 2009

PCR Amplification of Captured product

1 well (uL) X 6 1 well (uL) X 6
Template 10 Template
2X iProof Mastermix 50 300 2X iProof Mastermix 50 300
Amp F6.3 Sol (100uM) 0.4 2.4 Amp F6.3r Sol (100uM) 0.4 2.4
Amp R6.3Sol (100uM) 0.4 2.4 Amp R6.3r Sol (100uM) 0.4 2.4
50X SYBG I 0.8 4.8 50X SYBG I 0.8 4.8
H2O 31.2 187.2 H2O 31.2 187.2
Total Volume 90 540 Total Volume 90 540

98C 30S -> (98C 10S -> 60C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold