AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 31: Difference between revisions
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>Alan6017518 (New page: ==PCR Amplification of Captured product== {| {{table}} | ||1well (ul)||X6 |- | Template||10|| |- | 2X iProof Mastermix||50||300 |- | AmpF6.2SoL (10u0M)||0.4||2.4 |- | AmpR6.2Sol (100uM)||0...) |
>Alan6017518 |
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==PCR Amplification of Captured product== | ==PCR Amplification of Captured product== | ||
{| {{table}} | {| {{table}} | ||
| || | | ||1 well (uL)||X 6||||||1 well (uL)||X 6 | ||
|- | |- | ||
| Template||10|| | | Template||10||||||Template|||| | ||
|- | |- | ||
| 2X iProof Mastermix||50||300 | | 2X iProof Mastermix||50||300||||2X iProof Mastermix||50||300 | ||
|- | |- | ||
| | | Amp F6.3 Sol (100uM)||0.4||2.4||||Amp F6.3r Sol (100uM)||0.4||2.4 | ||
|- | |- | ||
| | | Amp R6.3Sol (100uM)||0.4||2.4||||Amp R6.3r Sol (100uM)||0.4||2.4 | ||
|- | |- | ||
| 50X SYBG I||0.8||4.8 | | 50X SYBG I||0.8||4.8||||50X SYBG I||0.8||4.8 | ||
|- | |- | ||
| H2O||31.2||187.2 | | H2O||31.2||187.2||||H2O||31.2||187.2 | ||
|- | |- | ||
| Total Volume||90 | | Total Volume||90||540||||Total Volume||90||540 | ||
|- | |- | ||
| | | | ||
|} | |} | ||
98C 30S -> (98C 10S -> | 98C 30S -> (98C 10S -> 60C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold |
Revision as of 21:14, 31 December 2009
PCR Amplification of Captured product
1 well (uL) | X 6 | 1 well (uL) | X 6 | |||
Template | 10 | Template | ||||
2X iProof Mastermix | 50 | 300 | 2X iProof Mastermix | 50 | 300 | |
Amp F6.3 Sol (100uM) | 0.4 | 2.4 | Amp F6.3r Sol (100uM) | 0.4 | 2.4 | |
Amp R6.3Sol (100uM) | 0.4 | 2.4 | Amp R6.3r Sol (100uM) | 0.4 | 2.4 | |
50X SYBG I | 0.8 | 4.8 | 50X SYBG I | 0.8 | 4.8 | |
H2O | 31.2 | 187.2 | H2O | 31.2 | 187.2 | |
Total Volume | 90 | 540 | Total Volume | 90 | 540 | |
98C 30S -> (98C 10S -> 60C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold