AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 31: Difference between revisions

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==TBE Gel==
==TBE Gel==
[[Image:ZhangLab_2 2010-01-04 12hr 26min.jpg]]

Revision as of 20:40, 4 January 2010

PCR Amplification of Captured product

NA12878 NA18507 NA20431 Jurkat New Jurkat Old

1 well (uL) X 6 1 well (uL) X 6
Template 10 Template
2X iProof Mastermix 50 300 2X iProof Mastermix 50 300
Amp F6.3 Sol (100uM) 0.4 2.4 Amp F6.3r Sol (100uM) 0.4 2.4
Amp R6.3Sol (100uM) 0.4 2.4 Amp R6.3r Sol (100uM) 0.4 2.4
50X SYBG I 0.8 4.8 50X SYBG I 0.8 4.8
H2O 31.2 187.2 H2O 31.2 187.2
Total Volume 90 540 Total Volume 90 540

98C 30S -> (98C 10S -> 60C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold

TBE Gel

File:ZhangLab 2 2010-01-04 12hr 26min.jpg