AlanFung:LabNotes/CTCF/2010-1-15: Difference between revisions

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>Alan6017518
(New page: ==Quantify Bisulfite Converted Samples with Qubit ssDNA Kit== ==On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP== 7 samples Prepare 7 Qubit Assay tubes Prepare 7*200ul dilute...)
 
>Alan6017518
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Line 4: Line 4:
  Prepare 7 Qubit Assay tubes
  Prepare 7 Qubit Assay tubes
   
   
  Prepare 7*200ul diluted dye=840ul
  Prepare 7*200ul diluted dye=1400ul
  Mix 4.2ul dye with 835.8ul buffer
  Mix 7ul dye with 1393ul buffer


  Mix 2ul samples with 98ul diluted dye
  Mix 2ul samples with 98ul diluted dye

Revision as of 16:27, 15 January 2010

Quantify Bisulfite Converted Samples with Qubit ssDNA Kit

On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP

7 samples
Prepare 7 Qubit Assay tubes

Prepare 7*200ul diluted dye=1400ul
Mix 7ul dye with 1393ul buffer
Mix 2ul samples with 98ul diluted dye
vortex spin down and wait for 2 mins

Result

PCR quantification of Bisulfite treated samples using 3 paris of CHR primers

On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP