AlanFung:LabNotes/CTCF/2010-1-15: Difference between revisions

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==PCR quantification of Bisulfite treated samples using 3 paris of CHR primers==
==PCR quantification of Bisulfite treated samples using 3 paris of CHR bilsulfite conversion primers==
 
==On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP==
==On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP==

Revision as of 16:28, 15 January 2010

Quantify Bisulfite Converted Samples with Qubit ssDNA Kit

On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP

7 samples
Prepare 7 Qubit Assay tubes

Prepare 7*200ul diluted dye=1400ul
Mix 7ul dye with 1393ul buffer
Mix 2ul samples with 98ul diluted dye
vortex spin down and wait for 2 mins

Result

PCR quantification of Bisulfite treated samples using 3 paris of CHR bilsulfite conversion primers

On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP