AlanFung:LabNotes/CTCF/2010-1-15: Difference between revisions
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>Alan6017518 |
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Result | Result | ||
{| {{table}} | |||
| Start (ng)||Sample Concentration ng/ul||Volume||Amount Recovered (ng)||Yield % | |||
|- | |||
| 50||1.7||20||34||68 | |||
|- | |||
| 10||0.25||20||5||50 | |||
|- | |||
| 5||0.13||20||2.6||52 | |||
|- | |||
| 1||0.26||20||5.2||520 | |||
|- | |||
| 0.5||N/A||20|||| | |||
|- | |||
| 0.1||N/A||20|||| | |||
|- | |||
| 0.05||N/A||20|||| | |||
|- | |||
| | |||
|} | |||
==PCR quantification of Bisulfite treated samples using 3 paris of CHR bilsulfite conversion primers== | ==PCR quantification of Bisulfite treated samples using 3 paris of CHR bilsulfite conversion primers== | ||
==On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP== | ==On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP== |
Revision as of 17:49, 19 January 2010
Quantify Bisulfite Converted Samples with Qubit ssDNA Kit
On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP
7 samples Prepare 7 Qubit Assay tubes Prepare 7*200ul diluted dye=1400ul Mix 7ul dye with 1393ul buffer
Mix 2ul samples with 98ul diluted dye vortex spin down and wait for 2 mins Result
Start (ng) | Sample Concentration ng/ul | Volume | Amount Recovered (ng) | Yield % |
50 | 1.7 | 20 | 34 | 68 |
10 | 0.25 | 20 | 5 | 50 |
5 | 0.13 | 20 | 2.6 | 52 |
1 | 0.26 | 20 | 5.2 | 520 |
0.5 | N/A | 20 | ||
0.1 | N/A | 20 | ||
0.05 | N/A | 20 | ||