AlanFung:LabNotes/CTCF/2010-1-15: Difference between revisions

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>Alan6017518
>Alan6017518
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  Result
  Result
{| {{table}}
| Start (ng)||Sample Concentration ng/ul||Volume||Amount Recovered (ng)||Yield %
|-
| 50||1.7||20||34||68
|-
| 10||0.25||20||5||50
|-
| 5||0.13||20||2.6||52
|-
| 1||0.26||20||5.2||520
|-
| 0.5||N/A||20||||
|-
| 0.1||N/A||20||||
|-
| 0.05||N/A||20||||
|-
|
|}
 
==PCR quantification of Bisulfite treated samples using 3 paris of CHR bilsulfite conversion primers==
==PCR quantification of Bisulfite treated samples using 3 paris of CHR bilsulfite conversion primers==


==On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP==
==On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP==

Revision as of 17:49, 19 January 2010

Quantify Bisulfite Converted Samples with Qubit ssDNA Kit

On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP

7 samples
Prepare 7 Qubit Assay tubes

Prepare 7*200ul diluted dye=1400ul
Mix 7ul dye with 1393ul buffer
Mix 2ul samples with 98ul diluted dye
vortex spin down and wait for 2 mins

Result
Start (ng) Sample Concentration ng/ul Volume Amount Recovered (ng) Yield %
50 1.7 20 34 68
10 0.25 20 5 50
5 0.13 20 2.6 52
1 0.26 20 5.2 520
0.5 N/A 20
0.1 N/A 20
0.05 N/A 20

PCR quantification of Bisulfite treated samples using 3 paris of CHR bilsulfite conversion primers

On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP