Human Chromosome Haplotyping: Difference between revisions

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The purpose of this experiment is to isolate individual chromosomes frozen in the metaphase state - where the DNA is condensed and ordered into discrete chromosomes - so that they may be separated out and analyzed. We hope to accomplish this by first culturing cell lines, then after they have grown enough, mixing colcemid, which will freeze the cells in the metaphase state, into the cell culture medium. After harvesting the cells exposed to colcemid, we use various methods to lyse the cells and separate out the DNA from the rest of the cell material.  
The purpose of this experiment is to isolate individual chromosomes frozen in the metaphase state - where the DNA is condensed and ordered into discrete chromosomes - so that they may be separated out and analyzed. We hope to accomplish this by first culturing cell lines, then after they have grown enough, mixing colcemid, which will freeze the cells in the metaphase state, into the cell culture medium. After harvesting the cells exposed to colcemid, we use various methods to lyse the cells and separate out the DNA from the rest of the cell material.  


==Procedures==
==Procedures and Experiments==


[[Chromosome Separation Protocol]]
[[Chromosome Separation Protocol]]
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[[Protease and Proteinase Trials]]
[[Protease and Proteinase Trials]]
[[Comparison of Staining Dyes]]


==Cell Culture Notes==
==Cell Culture Notes==

Revision as of 23:46, 27 April 2010

Background

The purpose of this experiment is to isolate individual chromosomes frozen in the metaphase state - where the DNA is condensed and ordered into discrete chromosomes - so that they may be separated out and analyzed. We hope to accomplish this by first culturing cell lines, then after they have grown enough, mixing colcemid, which will freeze the cells in the metaphase state, into the cell culture medium. After harvesting the cells exposed to colcemid, we use various methods to lyse the cells and separate out the DNA from the rest of the cell material.

Procedures and Experiments

Chromosome Separation Protocol

  • First procedure used

Chromosome Separation Trials

Proteinase K Preparation

Protease and Proteinase Trials

Comparison of Staining Dyes

Cell Culture Notes

<calendar> name=Kyle:Cell Culture Notes format=%name/%year-%month-%day date=2010/01/01 view=oneyear </calendar>